Experience with laser doppler flowmetry as an efficacy criterion for combination treatment using Intense Pulsed Light (IPL) technology, local ozonide therapy, and mesoozone therapy in patients with rosacea

Pershina K.S., Bitkina O.A., Kosheleva I.V., Peretyagin P.V., Bitkina E.V., Krapivina O.I.

1) Privolzhsky Research Medical University, Nizhny Novgorod, Russia; 2) Russian Medical Academy of Continuous Professional Education, Moscow, Russia; 3) National Research State University of Nizhny Novgorod named after N.I. Lobachevsky, Nizhny Novgorod, Russia; 4) Peoples’ Friendship University of Russia named after Patrice Lumumba, Moscow, Russia

Background: This study was aimed to the evaluation of the diagnostic value of laser Doppler flowmetry (LDF) and analysis of the dynamics of skin microcirculation in patients with rosacea who received various types of physiotherapy treatments: phototherapy, mesoozone therapy, and phototherapy using local ozone therapy in the form of topical ozonide-containing agents.
Objective: Evaluation of the diagnostic value of LDF in identifying skin microcirculation disorders in patients with rosacea and determination of the potential for its use in dynamically assessing the effectiveness of various therapeutic methods: phototherapy, mesoozone therapy, and a combination of phototherapy and local ozonide therapy.
Materials and methods: The study included 61 patients with the erythematotelangiectatic subtype of rosacea. Patients were divided into 3 groups: phototherapy, phototherapy combined with ozonide therapy, and mesoozone therapy. Microcirculation parameters were assessed using LDF during treatment.
Results: Variability in baseline microcirculation parameters was established. Improvements were noted in all groups. The most pronounced restoration of the endothelial component was observed with combination therapy (p<0.05). Phototherapy primarily affected the neurogenic component, while mesoozone therapy affected the bypass index.
Conclusion: LDF is an effective method for objectively assessing microcirculation and monitoring therapy for rosacea. Combined treatment methods demonstrate the greatest efficacy.

For citations: Pershina K.S., Bitkina O.A., Kosheleva I.V., Peretyagin P.V., Bitkina E.V., Krapivina O.I. Experience with laser doppler flowmetry as an efficacy criterion for combination treatment using Intense Pulsed Light (IPL) technology, local ozonide therapy, and mesoozone therapy in patients with rosacea. Pharmateca. 2026;33(5):110-120. (In Russ.). DOI: https://dx.doi.org/10.18565/pharmateca.2026.5.110-120

Authors’ contribution: The concept and design of the article – K.S. Pershina, O.A. Bitkina, I.V. Kosheleva, P.V. Peretyagin. Writing the text – K.S. Pershina, O.A. Bitkina, I.V. Kosheleva, P.V. Peretyagin, E.V. Bitkina, O.I. Krapivina. Editing – K.S. Pershina, O.A. Bitkina, I.V. Kosheleva, E.V. Bitkina.
Conflicts of interest: The authors confirm that they have no conflicts of interest to declare.
Funding: The study was conducted without any sponsorship.
Ethical Approval: The study was approved by the Ethics Committee of the Privolzhsky Research Medical University, Protocol No. 6 dated June 7, 2024.
Patient Consent for Publication: All patients provided informed consent for the publication of their data.
Authors’ Data Sharing Statement: The data supporting the findings of this study are available upon request from the corresponding author after approval from the principal investigator.

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About the Authors

Kristina S. Pershina, Postgraduate Student, Department of Skin and Venereal Diseases, Privolzhsky Research Medical University, Nizhny Novgorod, Russia; kris.tina1300@mail.ru, ORCID: https://orcid.org/0009-0003-4362-9618, eLibrary SPIN: 5989-4477 (corresponding author)
Oksana A. Bitkina, Dr. Sci. (Med.), Associate Professor, Professor at the Department of Skin and Venereal Diseases, Privolzhsky Research Medical University, Nizhny Novgorod, Russia; ORCID: https://orcid.org/0000-0003-4993-3269
Irina V. Kosheleva, Dr. Sci. (Med.), Professor, Professor at the Department of Dermatovenereology and Cosmetology, Russian Medical Academy
of Continuous Professional Education, Moscow, Russia; ORCID: https://orcid.org/0000-0003-2767-3021
Petr V. Peretyagin, Cand. Sci. (Biol.), Senior Researcher, Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia; ORCID: https://orcid.org/0000-0003-0707-892X
Elizaveta V. Bitkina, 6th-year student, Faculty of General Medicine, Peoples’ Friendship University of Russia named after Patrice Lumumba, Moscow, Russia; ORCID: https://orcid.org/0009-0009-3175-0836
Olga I. Krapivina, 5th-year student, Faculty of General Medicine, Privolzhsky Research Medical University, Nizhny Novgorod, Russia; ORCID:
https://orcid.org/0009-0005-7951-3480

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