An innovative method of pregravid preparation of the “thin” endometrium during IVF

Shneiderman M.G., Kalinina E.A., Smolnikova V.Yu., Alieva K.U., Aksenenko A.A., Kuzemin A.A., Dyuzheva E.V., Gavisova A.A., Fatkhudinov T.Kh., Boris D.A., Afyan A.I., Apolikhina I.A.

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

Background: In the context of declining fertility and in order to increase the effectiveness of the in vitro fertilization method when diagnosed with hypoplastic (thin) endometrium, the development of a new method of treating this pathology becomes relevant.
Objective: Development of a new effective method of treating hypoplastic (thin) endometrium without the use of standard treatment methods - hormone therapy and physiotherapy used in preparing patients for in vitro fertilization (IVF), and evaluation of the effectiveness and safety of the proposed innovative method.
Material and methods: The study included 260 patients of reproductive age (from 25 to 48 years) with diagnosed primary or secondary infertility and the presence of “thin endometrium” that was not treatable by other methods and did not respond to hormonal stimulation carried out in cycles three or four months before the start of the study. In the control group, 30 women with a clinically confirmed diagnosis of “infertility 1 or infertility 2, thin endometrium” were examined. The procedure for irrigation of the endometrium with carbon dioxide was carried out three times on the 7th, 9th and 11th days of the menstrual cycle. Statistical data processing was carried out using Statistica 10.0 software packages.
Results: After the patients underwent three irrigations of the endometrium with carbon dioxide, there was a clear trend towards an increase in the thickness of the endometrium. This indicates the potential of the method in increasing the number of successful embryo implantations during the IVF procedure.
Conclusion: Our own clinical observations accumulated confirm the high efficiency of using carbon dioxide (CO2) for irrigation of the uterine cavity with thin endometrium, which contributes to a significant increase in the endometrium thickness in the preparatory period before the IVF procedure.

For citations: Shneiderman M.G., Kalinina E.A., Smolnikova V.Yu., Alieva K.U., Aksenenko A.A., Kuzemin A.A., Dyuzheva E.V., Gavisova A.A., Fatkhudinov T.Kh., Boris D.A., Afyan A.I., Apolikhina I.A. An innovative method of pregravid preparation of the “thin” endometrium during IVF. Pharmateca. 2025;32(9):101-107. (In Russ.). DOI: https://dx.doi.org/10.18565/pharmateca.2025.9.101-107

Authors’ contribution: Concept and design of the study – Shneiderman M.G., Kalinina E.A., Smolnikova V.Yu. Working with patients and conducting medical procedures – Alieva K.U., Aksenenko A.A., Kuzemin A.A., Dyuzheva E.V., Gavisova A.A., Afyan A.I., Shneiderman M.G., Apolikhina I.A. Working with laboratory animals – Fatkhudinov T.Kh. Collection and processing of material – Afyan A.I., Shneiderman M.G., Boris D.A. Research supervision – Kalinina E.A.
Conflicts of interest: The authors confirm that they have no conflicts of interest to declare.
Funding: The study was conducted without any sponsorship.
Ethical Approval: The study protocol received approval from the local ethics committee of the V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology.
Patient Consent for Publication: The retrospective design of the study did not require informed consent from patients for statistical processing of anonymized data for subsequent publication.
Authors’ Data Sharing Statement: The data supporting the findings of this study are available upon request from the corresponding author after approval from the principal investigator.

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About the Authors

Mikhail G. Shneiderman, Cand.Sci. (Med.), Obstetrician-Gynecologist, V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; innamike@lmi.net
Elena A. Kalinina, Dr. Sci. (Med.), Head of the Department of Assisted Technologies in Infertility Treatment, Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; e_kalinina@oparina4.ru
Veronika Yu. Smolnikova, Dr. Sci. (Med.), Leading Researcher, Department of Assisted Technologies in Infertility Treatment, Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia
Kamila U. Alieva, Cand. Sci. (Med.), Researcher, Department of Assisted Technologies in Infertility Treatment, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; kaya79@inbox.ru
Artem A. Aksenenko, Cand. Sci. (Med.), Obstetrician-Gynecologist, 1st Gynecology Department, V.I.Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; a_axenenko@oparina4.ru
Andrey A. Kuzemin, Cand. Sci. (Med.), Head of the Day Hospital, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia
Elena V. Dyuzheva, Cand. Sci. (Med.), Obstetrician-Gynecologist, Department of Assisted Technologies in Infertility Treatment, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; dug79@yandex.ru
Alla A. Gavisova, Dr. Sci. (Med.), Head of the 1st Gynecological Department, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; gavialla@gmail.com
Timur Kh. Fatkhudinov, Dr. Sci. (Med.), Professor, Leading Researcher, Laboratory of Regenerative Medicine, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; tfat@yandex.ru
Armine I. Afyan, Obstetrician-Gynecologist, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; armineafyan@mail.ru
Dayana A. Boris, Cand. Sci. (Med.), Obstetrician-Gynecologist, Ultrasound Physician, Researcher at the Department of Aesthetic Gynecology and Rehabilitation, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; dayana_boris@mail.ru, ORCID: https://orcid.org/0000-0002-0387-4040, SPIN: 4832-0643 (corresponding author)
Inna A. Apolikhina, Dr. Sci. (Med.), Professor, Honored Doctor of the Russian Federation, Head of the Department of Aesthetic Gynecology and Rehabilitation, V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Moscow, Russia; apolikhina@inbox.ru

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